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Constitutive and inducible pectinolytic enzymes from Aspergillus flavipes FP-500 and their modulation by pH and carbon source BJM
Martínez-Trujillo,Aurora; Aranda,Juan S.; Gómez-Sánchez,Carlos; Trejo-Aguilar,Blanca; Aguilar-Osorio,Guillermo.
Growth and enzymes production by Aspergillus flavipes FP-500 were evaluated on pectin, polygalacturonic acid, galacturonic acid, arabinose, rhamnose, xylose, glycerol and glucose at different initial pH values. We found that the strain produced exopectinases, endopectinases and pectin lyases. Exopectinases and pectin lyase were found to be produced at basal levels as constitutive enzymes and their production was modulated by the available carbon source and pH of culture medium and stimulated by the presence of inducer in the culture medium. Endo-pectinase was basically inducible and was only produced when pectin was used as carbon source. Our results suggest that pectinases in A. flavipes FP-500 are produced in a concerted way. The first enzyme to be...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus flavipes; Pectinolytic enzymes; Constitutive enzymes; Inducible enzymes; Pectin.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100006
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Kinetic study on inducibility of polygalacturonases from Aspergillus flavipes FP-500 Electron. J. Biotechnol.
Martínez-Trujillo,Aurora; Aranda,Juan S; Aguilar-Osorio,Guillermo.
The aim of this work was to describe growth dynamics, substrate depletion and polygalacturonases production by Aspergillus flavipes FP-500 in batch cultures by means of unstructured models. The microorganism was cultivated on several mono- di- and poly- saccharides, and then the culture development modeled with Monod and Leudeking-Piret equations. The kinetic parameters related to the models (µmax, γx/s, α and β) were obtained by minimizing the quadratic residuals function with a simplex algorithm. An accurate description of experimental data was attained with the proposed models. Besides, modeling provided significant kinetic information on microbial degradation of complex substrates, such as the correlation between specific...
Tipo: Journal article Palavras-chave: Constitutive enzymes; Inducible enzymes; Initial pH; Kinetic modeling; Pectinases.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400008
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